Efeitos de galactanas sulfatadas das algas marinhas Botryocladia occidentalis, Champia feldmannnii e Solieria filiformis no processo inflamatório agudo e na nocicepção. / Effects of sulfated galactans from marine algae Botryocladia occidentalis, Champa and Soli feldmannnii filiformis in acute inflammation and nociception.

Daniel Magalhães Gomes

The sulfated polysaccharides (SPL) constitutes a class of carbohydrates rich in polyanions and that presents a diversity of important biological activities. The antithrombotic and the anticoagulant activities are the most studied. It was demonstrated by the sulfated polysaccharides (SPL) the anti-inflammatory activity both in vitro and in vivo. However, the majority of the activities of these molecules demonstrated in literature are related to SPL obtained from brown seaweed (Phaeophytas) being these activities little exploited from the SPL obtained from other groups of seaweed. Moreover, the studies little explore the activity of these molecules in vivo and its pro-inflammatory properties. So, the present study has investigated the anti-inflammatory and pro-inflammatory activities of the SPL extracted from the red algae Botryocladia occidentalis, Solieria filiformes and Champia feldmannii, studing in more detail the pro-inflammatory activity of the latter besides its antinociceptives properties. Wistar rats (150-250 g) were used on the pro and anti-inflammatory studies and male Swiss mice (20-35 g) in the nociception studies. In the investigation of anti-inflammatory activity of these molecules, the animals of each group were injected by endovenous via (0.09 mg/kg) of their respectives SPL before they receive the inflammatory stimuli carrageenan, dextran or zimosan. The polysaccharides of B. occidentalis and S. filiformes, tested in the carrageenan oedema and dextran were not able to inhibit the formation of these oedema, while the SPL of C. feldmannii than not inhibit these oedema also not inhibited that elicitated by zimosan. In pro-inflammatory activit research the SPL were injected by subcutaneously intraplantar via. All SPL tested induced paw oedema, being the oedema of C. feldmannii the sharpest of all. During the investigation of pro-inflammatory properties of sulfated polysaccharides of C. feldmannii there was no correlation in studies of dose-response being the oedema of these molecules persistent for 24 hours after its application. Even the studies that prove their pro-inflammatory properties, these SPL also led neutrophils migration of abdominal cavity of rats also raising the vascular permeability in paw tissue of these animals. In attempts of modulation of this activity the animals received the SPL (0.9 mg/kg, sc) or 1 hour after the dexamethasone injection (1.0 mg/Kg, s.c.), indomethacin (5 mg/Kg, s.c.), L-NAME (25 mg/Kg; e.v), meclyzine (40 mg/kg, s.c.), pentoxifylline (90 mg/Kg, s.c.). The results demonstrated that only LNAME failed to inhibit the paw oedema elicitated by C. feldmannii, indicating, however, the possibility to have greater participation of cytokines and prostaglandins in the process. The nonciceptive activity of C. felmanni SPL as antinociceptiva was confirmed when its administration was done of (0.2, 1, 5 and 25 mg/kg, s.c.) 30 minutes before the injection of acetic acid 0.6%. The dose of 1 mg/kg presented the best inhibition. In short, the SPL testes demonstrated no posses anti-inflammatory activity, while the SPL of C. feldmannii showed a significant pro-inflammatory activity in addition to its potent nonciceptive activity.

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