Efeitos da administração de corticosteroide sobre o intestino de ratos submetidos a gastrosquise experimental intra-utero : avaliação morfologica, histologica e bioquimica

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Background: Gastroschisis is the congenital anomaly of the abdominal wall closure. The eviscerated bowel is exposed to the amniotic fluid, which leads to an intestinal inflammatory reaction. The goal in the treatment of gastroschisis is to prevent intestinal injury, as well as the motility, absorptive and infectious complications. Purpose: The author evaluated the effects of maternal corticosteroid administration on the intestines of rats submitted to fetal gastroschisis, through morphometrical, histological and biochemical analysis. Methods: A fetal gastroschisis rat model was used. Pregnant rats were submitted to surgery on day 18.5 of pregnancy (term = 22 days). Two groups were assessed: with and without maternal dexamethasone adnmristration. Each group was composed by gastroschisis, control and sham fetuses. Morphometry: Fetal body weight, intestinal weight and intestinal length were assessed. Histology: Analysis involved measures of intestinal loop diameter, total intestinal wall, mucosal and submucosal, both circular and longitudinal muscles layers and serosal thickness. Biochemics: The intestinal cytokine profile: EL-1, IL-6, IL-10, tumor necrosis factor, gamma- interferon and protein content were assessed. Differences between groups and subgroups were tested by the ANOVA method with a significant p value <0.05. Results: Dexamethasone promoted a decrease in all the morphometric data, except in the intestinal length. There was a decreased intestinal protein content in gastroschisis fetuses. Dexamethasone increased the intestinal protein content in gastroschisis, control and sham fetuses. In both groups, all histological parameters were increased in gastroschisis fetuses (p<0.0001). It could not be identified a particular profile of cytokine for gastroschisis fetuses. Conclusions: Rat fetuses submitted to experimental gastroschisis had decreased body weight and increased intestinal weight. Histological analysis demonstrated that the increased intestinal weight was related to an increased thickness of all intestinal wall layers in gastroschisis fetuses. Dexamethasone triggered a substantial decrease in intestinal weight, and increased the intestinal protein content in gastroschisis fetuses. It may be useful in managing the intestinal damage in gastroschisis

ASSUNTO(S)

feto - cirurgia corticosteroides citocinas gastrosquise feto - doenças

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