Efeito do tratamento anti-inflamatÃrio na histologia endometrial, produÃÃo de prostaglandina e taxa de gestaÃÃo apÃs transferÃncia de embriÃes e/ou manipulaÃÃo cervical em Ãguas / Efeito do tratamento anti-inflamatÃrio na histologia endometrial, produÃÃo de prostaglandina e taxa de gestaÃÃo apÃs transferÃncia de embriÃes e/ou manipulaÃÃo cervical em Ãguas / Effect of anti inflammatory treatment in endometrial histology, prostaglandin production and pregnancy rates after embryo transfer and/or cervical manipulation in mares / Effect of anti inflammatory treatment in endometrial histology, prostaglandin production and pregnancy rates after embryo transfer and/or cervical manipulation in mares

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Embryo transfer is currently the main breeding tool in equine reproduction. However it is still necessary to improve pregnancy to lower its costs.Among many factors that influence ET results, recipient mare management should be underlined. Currently the method utilized is non surgical transcervical transfer. Equine ET professionals observed that the less cervical manipulation in recipients during transfer the higher the pregnancy rates. These results are probably related to prostaglandin production stimulus or endometritis post genital tract manipulation. The main objective of this study is to evaluate the association between prostaglandin F2α (PF2α) release and endometritis with cervix manipulation and the embryo implanting rod passage through the cervix. It was also investigated whether the anti inflammatory drug, Flunixin Meglumine is efficient in the inhibiting of PGFM production and endometritis, thus, associated with better recipient pregnancy rates. In this study, 209 mares were used. In experiment control mares had higher PGFM concentrations after cervix manipulation and rod passage compared to FM injected mares which received injections at 6, 3 and zero hours in relation to the cervix stimulus. In the second experiment, 42 mares were allocated randomly to one of four treatments before slaughter: G1 â no manipulation and no FM injection; G2 â cervix manipulation and no FM; G3 â cervix manipulation and FM injection (1.1 mg/kg of body weight) and G4 â no manipulation and FM injection. More G2 and G3 mares had endometritis. However, nine G3 mares (81.8%) had inflammatory infiltration in the endometrium, despite having received FM. It was not possible to establish if the endometritis were caused by cervix manipulation. The majority of alterations were inflammation (57.57%), atrophy (37.37%) and endometrial fibrosis (27.57%). In the third trial FM lowered recipient pregnancy rates. An association between cervix manipulation and PGFM production was found. Variations on PGFM concentrations associated to the passing of an embryo implantation rod were discrete. There was no apparent pattern in the association between FM injection and PGFM production, as no effect of FM on the inhibition of endometritis was observed. Under the conditions of this study the utilization of FM was deleterious to reproduction and its use with the objective of improving pregnancy rates in mares should be discouraged.

ASSUNTO(S)

Ãgua flunexin meglumini fertilidade zootecnia prostaglandina f@ α

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