Efeito do selenito de sodio na reparação ossea em tibias de ratos irradiados

AUTOR(ES)

Anna Silvia P. Setti da Rocha

DATA DE PUBLICAÇÃO

2005

RESUMO

Considering that the ionizing radiation may cause deleterious effects on the process of tissue repair and sodium selenite is an antioxidant agent, acting against the free radicals in the organism, this study aimed at evaluating the radioprotective effect of sodium selenite on the process of bone repair in tibiae of Wistar rats. The sample comprised 100 rats and was divided into four experimental groups: control, irradiated, selenium and selenium/irradiated. Ali animals were submitted to a surgical procedure for production of a bane defect in the right and left tibiae. The animals in the selenium and selenium/irradiated groups received a single dose of sodium selenite (1 mg/kg of body weight) via intraperitoneal injection; the animals in the selenium/irradiated group received the injection 15 hours before irradiation. The irradiation for the irradiated and selenium/irradiated groups was applied with a cobalt therapy machine (CO60) with simple dose at 8 Gy on the lower limbs, three days after surgery. After 7, 14, 21, 28 and 45 days, the process of one repair was morphologically evaluated by Hematoxylin Eosin and Mallory Trichrome staining; ultrastructural analysis by scanning electron microscopy; and quantitatively evaluated by the volumetric density. As to morphology, it was observed that the selenium/irradiated group at 7 days presented a Iate repair compared to the control group; however, at 28 days, the control, selenium and selenium/irradiated groups presented a similar pattern of bone repair, which was also revealed by scanning electron microscopy at 45 days. Quantitatively, there was a statistically significant difference between the means of volumetric density for the selenium and seleniumlírradiated groups at 7, 14 and 28 days and between the control and selenium groups at 14 days. Thus, it was concluded that sodium selenite, despite being toxic at the seventh day of tissue repair, was an effective radioprotective agent for bone repair in tibiae of rats

ASSUNTO(S)

ossos - crescimento glutationa peroxidase osteogenesis selenium selenio glutathione peroxidase

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