Efeito do Ãcido barbÃtico de Cladomia salzmannii (Nyl.) nanoencapsulado sobre Trypanosoma cruzi

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The aim of this study was to evaluate the effects of barbatic acid (BARB) on the viability, growth and ultrastructure of Trypanosoma cruzi epimastigote forms, the ethiologic agent of Chagasâ disease. In order to improve the BARB bioavailability this compound was incorporated into D,L-(lactide-glycolide) copolymer (PLGA) nanocapsules (BARB-NC). The cell growth and viability was evaluated by cell counting using Neubauer chamber for 24, 48 and 72 hours. The parasites were incubated at different BARB or BARB-NC concentrations for 72 hours estimated after 24 hours of cultivation. Parasites grown in drug free medium were used as a control. The incubation of parasites with unloaded nanocapsules resulted in no inhibitory activity in comparasion with control cells, but slight ultrastructural changes were verified. Treatment of parasites with both BARB and BARB-NC resulted in a dose-dependent growth inhibition. BARB caused an inhibition of only 32% of parasite proliferation at 62.5 μg/ml at 24 hours. However, the IC50 value of BARB-NC at 24 hours was 24.66 μg/ml. The incorporation of BARB into nanocapsules enhanced therefore its trypanocidal activity. Nevertheless the treatment of epimastigotes with both BARB and BARB-NC caused drastic morphological changes with a number of affected cells and severe ultra-structural alterations considerably higher in BARB-NC treated parasites

ASSUNTO(S)

trypanosoma cruzi trypanosoma cruzi bioquimica barbatic acid nanocapsules nanocÃpsulas Ãcido barbÃtico

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