DsRed2 transient expression in Culex quinquefasciatus mosquitoes
AUTOR(ES)
Wilke, Andre Barretto Bruno, Scaife, Sarah, Alphey, Luke, Marrelli, Mauro Toledo
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
2013-06
RESUMO
Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions.
Documentos Relacionados
- Controle genético de mosquitos Culex quinquefasciatus
- Red Fluorescent Protein (DsRed) as a Reporter in Saccharomyces cerevisiae
- Identification of different emitting species in the red fluorescent protein DsRed by means of ensemble and single-molecule spectroscopy
- Transformation of Fusarium oxysporum f sp. vasinfectum with GFP and DsRed gene markers.
- Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-Å resolution