Controle genético de mosquitos Culex quinquefasciatus / Genetic control of Culex quinquefasciatus mosquitoes

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The Culex quinquefasciatus mosquitoes are considered urban plague and are able to grown in polluted water achieving high density. This specie are important vectors in the transmission of parasites and arbovirus. Chemical control methods shown to be ineffective and highly hazards to the environment. In this way, new technologies of control have been developed, among them there is SIT (Sterile Insect Technique) that utilizes radiation to sterilize males and then release in the environment to mate with wild females. New genetic methods are been proposed based on the SIT technique, they relies on the integration of a dominant lethal gene associated to a specific promoter of female avoiding the need for radiation (RIDL). In this process the insects receive diet supplemented with a chemical repressor (tetracycline). The expression of the dominant lethal gene is kept off while this repressor is added to the larvae. For the samples that would be being prepared for release, the repressor is removed, and the dominant lethal gene is activated, causing the death of all the females, and consequently only leaving male for release. The homozygous males for the lethal gene would be set free to mate with the wild females. The lineages would be heterozygous for the lethal gene and only males would survive. Since that, the capacity of mating of the transgenic males produced by method RDIL is an important prerequisite for the success of the program. It was crucial to achieve the objectives of this project adapt the microinjection method to Culex quinquefasciatus, so it became possible inject the LA513, LA882 and LA3653 plasmids seeking for the transgenic transformation. The obtaining of the transgenic mosquito Culex quinquefasciatus seems to be more difficult then expected and its development was harmed. The practical applications using RIDL in vector control is huge and can become a useful tool in the Integrated Management of vectors.

ASSUNTO(S)

transgenics culex quinquefasciatus ridl culex quinquefasciatus control ridl transgênicos controle

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