Doenças de Newcastle: padronização de testes sorológicos para o diagnostico em avestruzes (Struthio Camelus) e avaliação soroepidemiológica nos Estados da Bahia e de São Paulo.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Newcastle Disease is a highly contagious viral infection, which attacks several avian species, and represents one of the most important diseases for the modern poultry industry. Because of the relevance of the poultry industry in Brazil and the boom in ostrich rearing, it?s necessary the development of control strategies and it depends on the development of practical and reliable tools for diagnosis. Such tools are available for chickens; however specific tests were not developed for ostriches yet. The aim of the present work is to standardize serological tests for the detection of antibodies against the Newcastle disease in ostriches and assess the seroepidemiological situation of flocks located in Bahia and São Paulo. The standardization of haemagglutination inhibition (HI) revealed interference of the type of used red blood cells and demonstrated the need of the use of erythrocytes of the same species or, alternatively, of turkeys. Indirect ELISA were developed or modified for this species and, in spite of they present high correlation amongst themselves, they demonstrated low correlation with HI. "Western blot" and "dotblot" were developed, and that can be useful in evaluation of the immune response and to make possible the implantation of control programs. The samples analyzed revealed the presence of antibodies against NDV and, as these animals were not vaccinated, the hypothesis that the ostriches are in contact with the vaccine virus or wild virus is reinforced.

ASSUNTO(S)

ciências biológicas elisa ostriches western-blott western-blot dot-blot avestruzes newcastle disease dot-blot doença de newcastle inibição da hemaglutinação elisa hi

ACESSO AO ARTIGO

Documentos Relacionados