Differential expression analysis between merozoites and sporozoites of Eimeria tenella using LongSage. / Análise da expressão diferencial entre merozoítos e esporozoítos de Eimeria tenella empregando a técnica de LongSAGE.

AUTOR(ES)

Jeniffer Novaes Gonçalves Dias

DATA DE PUBLICAÇÃO

2009

RESUMO

Eimeria tenella is one of the most important causing agents of poultry coccidiosis. To study the quantitative gene expression profile in zoite stages of LongSage libraries were generated from merozoites and sporozoites. More than 35.000 tags were obtained, whose 9.516 were unique. For identification and annotation of differential expressed genes, tags were extracted, counted and submitted to statistical analysis by Sage Analysis, software developed by our group. A total of 197 tags were reconstructed and automatic annotated. Stage-specific expression genes and distinct transcriptional profile were observed between these stages. In merozoites the results were related to protein translation and folding, and in sporozoites the proteins were involved to chromatin structure, transport and catalytic activity. To LongSAGE validation, differential expression was quantified using RT-qPCR to a small group of genes. Good correlation was observed between these platforms.

ASSUNTO(S)

eimeria tenella coccidiose gene expression sporozoites merozoites eimeria tenella merozoítos coccidiosis expressão gênica esporozoítos

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