Determinação dos óxidos de colesterol em pacientes diabéticos e intolerantes à glicose / Cholesterol oxides as biomarkers of oxidative stress in type 1 and type 2 diabetes mellitus

AUTOR(ES)

Simone Ferderbar

DATA DE PUBLICAÇÃO

2004

RESUMO

Oxidative stress can play an important role in the etiology of the complications of diabetes mellitus. The increase in the production of oxidant species promotes alterations in endogenous molecules, including cholesterol. Cholesterol oxides (COx) are formed by the oxidation of cholesterol by enzymatic processes or by processes involving free radicals. They present important biological effects that can contribute to the development of the atherosclerotic process in diabetes. In this study, the concentrations of the COx in diabetic patients and individuals who are intolerant to glucose was determined in order to establish whether the Cox are sensitive markers of lipoperoxidation in glucose intolerance and diabetes. Serum concentrations of the COx were determined by GC-FID in the following groups: Type 1 diabetics (DM1), type 2 diabetics (DM2), patients intolerant to glucose (IGT) and normoglycemic subjects (controls). The concentrations of total cholesterol oxides were found to be elevated in the DM1 and DM2 groups with respect to the normoglycemic subjects (p<0.05). The serum concentrations of 7⓹- hydroxicholesterol (7α-OH), 7β-hydroxicholesterol (7&#$946;-OH) and 25-hydroxicholesterol (25-OH) were found to be increased in the DM1 group with respect to the DM2 group (p<0.05). The comparison between the control, IGT and DM2 groups indicated a significant increase in the concentrations of 7β-OH, cholesterol-β-epoxide and cholesterol-α-epoxide in the DM2 group (p<0.05). In conclusion, cholesterol oxides could serve as suitable biomarker of lipoperoxidation to indicate the intensity of lipid oxidative mofications in diabetic patients.

ASSUNTO(S)

diabetes mellitus bioquímica clínica clinical biochemistry estresse oxidativo oxidative stress Óxidos de colesterol oxysterols diabetes mellitus

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