Detection of N-(3-Oxohexanoyl)-l-Homoserine Lactone in Mice Infected with Yersinia enterocolitica Serotype O8

AUTOR(ES)

Jacobi, Christoph A.

FONTE

American Society for Microbiology

RESUMO

Yersinia enterocolitica synthesizes N-acyl-l-homoserine lactone (AHL) signal molecules via the LuxR-LuxI homologues YenR-YenI. In this study we checked two prototypes of mouse-virulent Y. enterocolitica serotype O8 strains WA-314 and 8081 for AHL production in vitro and in vivo (mouse infection model). We used thin-layer chromatography in combination with the Escherichia coli AHL biosensor to identify the AHL species produced. We detected only OHHL [N-(3-oxohexanoyl)-l-homoserine lactone] and not HHL (N-hexanoyl-l-homoserine lactone) produced by Y. enterocolitica O8 in culture supernatant or infected mouse tissue. This is the first report demonstrating AHL production by yersiniae during infection.

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