Detecção e caracterização molecular de sapovírus em suínos assintomáticos

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

28/03/2011

RESUMO

Porcine sapovirus (PoSaVs) infections are frequent in pig herds in America, Europe and Asia. The infection can be symptomatic, in which the typical clinical signs is diarrhea. However, asymptomatic infections are described with relative frequency. The genetic diversity of the PoSaV is high. Five genogroups and 16 genotypes are well established and five genogroups are being proposed in field strains. The aim of this study was to verify epidemiological and molecular characteristics of the PoSaV infection in asymptomatic pigs. A total of 169 fecal samples were randomly collected from 14 commercial pig herds (five breeding herds without growing pigs and nine growing-pig herds) from Western Parana state, Brazil. Fecal samples from nursing piglets (n=20), post-weaning pigs (n=37), finisher pigs (n=71), and breeding animals (n=41) were collected. The presence of PoSaV in fecal samples was evaluated by a RT-PCR assay with primers targeting the 331 bp product from the polymerase viral gene. PoSaV was detected in 10 (71.4%) of the pig farms included in this study. All of five breeding herds without growing pigs and 5 (55.5%) of the growing-pig herds had animals shedding PoSaV in the feces. The RT-PCR assay was positive in 40 (23.7%) out of 169 fecal samples evaluated. The rates of positive samples in animal category range from 17.1 to 40.5%. The infection was associated with animal category, being more frequent in post-weaning pigs (P<0.05). The phylogenetic analysis carried out in three Brazilian PoSaV strains allowed the classification of one strain in the genogroup III genotype 3 and two other strains in the recently identified genogroup IX, never before described in the American continent. The results showed that the PoSaV infection could be widely disseminate in Brazilian pig herds, even in healthy animals and that the infection is more frequent in weaned pigs in the nursery. The Brazilian PoSaV field strains presented genetic diversity.

ASSUNTO(S)

suíno - doenças viroses em animais virologia veterinária suíno - infecção suíno - viroses swine diseases virus diseases in animals veterinary virology

ACESSO AO ARTIGO

Documentos Relacionados