Culture environment in the micropropagation of Annona glabra L. / "Ambiente de cultivo na micropropagaÃÃo de Annona glabra L."

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The Annona glabra L. has aroused a great interest in Brazil in recent years, since it is a species with the capacity to adapt to several environments and it belongs to the fruit group which has medicinal properties of substances isolated from leaves, fruit and seeds. The initiatives to explore the potential of this species are incipient still, especially due to the difficulty to induce multi-shooting on explants, besides the shortage of information about the culture systems. Among the factors which can affect the conditions of the culture environment, the type of seal stands out. Different in vitro methods have been used to investigate the direct effects on the closure system. The objectives of this work were to study: 1) the effects of the culture environment on the physiology and morphology of micropropagated Annonas glabra L. plants, verifying the explants and/or plant behavior on in vitro culture environment, as well as the influences of different vessel seals and 2) the photosynthetic characteristics of micropropagated plants. Two different factors were tested: environment (conventional growth room and greenhouse) and closure systems of the culture container [transparent plastic caps and seal with parafilm, which restrict the exchange of gases between the container and the outside atmosphere (conventional system) and modified plastic caps, containing a pair of orifices (10 mm diameter) with a membrane filter pair permeable to gases]. The natural light, under the closure system with a modified cap, result in an increase of the amount of shoots, alterations in the chlorophyll content, photosynthetic potentials and morphological characteristics of plants from Annona glabra L. The evaluated plants present adaptability to the culture conditions and photosynthetic characteristics to the in vitro propagation. The culture environment influence on the species physiology and anatomy, implied in the reduction of the necessity of cares during the transference of these plants to greenhouse condition. The process of acclimatization allowed the survival of micropropagated plants to ex vitro conditions.

ASSUNTO(S)

growth regulators cultura de tecidos ecofisiologia reguladores de crescimento tissue culture annona glabra l. annona glabra l. natural light plant anatomy anatomia vegetal luz natural fisiologia de plantas cultivadas ecophysiology

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