Cultivo de embriÃes imaturos provenientes de hibridaÃÃo controlada entre PÃra Rio e Poncà . / Cultivo de embriÃes imaturos provenientes de hibridaÃÃo controlada entre PÃra Rio e Poncà .

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The aim of this research was to study the embryonic development at different stages, the influence of diverse concentrations of MT medium (Murashige &Tucker, 1969), sucrose, vitamins, activated charcoal and gibberellic acid on the culture of immature embryos from cross between âPÃra Rioâ x âPoncÃâ. The embryos were excised under aseptic conditions and inoculated in 15 mL of MT according to each of the following experiments: 1) stages of development (globular, heart-shaped and cotiledonary) associated with concentrations of 0%, 50%, 100% and 150% of the MT medium; 2) MT concentrations (0%, 50%, 100%, 150% and 200%) combined with 0, 30, 60 and 90 g.L-1 of sucrose; 3) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) combined with 0, 30, 60 and 90 g.L-1 of sucrose; 4) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) combined with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the inoculation, the embryos were maintained for 90 days in growth room at 27Â1ÂC temperature, 16-hour photoperiod and 32 umol.m-2.s-1 of irradiance. High development was obtained with embryos at cotiledonary stage. High development at the globular embryos stage were obtained using 50% of the MT added to 60 g.L-1 of sucrose and 100% of the same medium added to 90 g.L-1 of sucrose. Both supplemented with 0,01 mg.L-1 of GA3. There was no need to add activated charcoal and vitamins in the MT to globular embryos culture.

ASSUNTO(S)

irradiance embryos culture fitotecnia cultura de tecidos biotecnologia poncà citros

ACESSO AO ARTIGO

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