Comparison and evaluation of colorimetric methods used to proteins determination in waste stabilization ponds / Comparação e avaliação dos métodos colorimétricos utilizados para determinação de proteínas em lagoas de estabilização

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Stabilization ponds represent a simple method to treat sanitary sewage. A large part of the organic matter present in sanitary sewage consists of proteins, which become a limiting factor for the wastewater treatment in biological processes. The goal of this work was to evaluate and to compare colorimetric methods for protein determination, defining which is the more appropriate for this environment. The colorimetric methods used were the Folin-phenol, Bradford, Biuret and Bicinconinic Acid methods. The protein standards used were serum albumin bovin, casein and ovalbumin. The Bradford method introduced several disadvantages. Biuret I and III and brute protein introduced high values due don t measure only proteins. The proteins quantity in the sample wasn t enough so that it had occurred reaction in the Biuret II method. The method of Bicinconinic Acid didn t react with casein and the reagent cost is high. Therefore, the method appropriated for this sample was the Lowry that introduced larger reprodutivity and sensitivity. To prove this result, a standard addition analysis was performed to verify if there were interfering substances, whose result was negative

ASSUNTO(S)

protein proteínas stabilization ponds lagoas de estabilização colorimetrics methods métodos colorimétricos

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