Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis.
AUTOR(ES)
Pavelka, M S
RESUMO
Diaminopimelate (DAP) is used by bacteria for the synthesis of lysine. In many species of bacteria, including mycobacteria, DAP is also used for peptidoglycan biosynthesis. In this report we describe the cloning of the dapB gene encoding dihydrodipicolinate reductase (DHPR), which catalyzes a key branch point reaction in the bacterial DAP biosynthetic pathway, from Mycobacterium tuberculosis. Analyses of the DapB proteins from different bacterial species suggest that two different classes of DHPR enzymes may exist in bacteria.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=179033Documentos Relacionados
- A cluster of three genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum encodes dihydrodipicolinate synthase, dihydrodipicolinate reductase, and a third polypeptide of unknown function.
- Cloning and characterization of the aroA gene from Mycobacterium tuberculosis.
- The rpoB gene of Mycobacterium tuberculosis.
- Characterization of the norB Gene, Encoding Nitric Oxide Reductase, in the Nondenitrifying Cyanobacterium Synechocystis sp. Strain PCC6803
- Nucleotide sequence of the 19 kDa antigen gene from Mycobacterium tuberculosis.