Cloning and characterization of the aroA gene from Mycobacterium tuberculosis.

AUTOR(ES)

Garbe, T

RESUMO

The aroA gene from Mycobacterium tuberculosis has been cloned by complementation of an aroA mutant of Escherichia coli after lysogenization with a recombinant DNA library in the lambda gt11 vector. Detailed characterization of the M. tuberculosis aroA gene by nucleotide sequencing and by immunochemical analysis of the expressed product indicates that it encodes a 5-enolpyruvylshikimate-3-phosphate synthase that is structurally related to analogous enzymes from other bacterial, fungal, and plant sources. The potential use of the cloned gene in construction of genetically defined mutant strains of M. tuberculosis by gene replacement is proposed as a novel approach to the rational attenuation of mycobacterial pathogens and the possible development of new antimycobacterial vaccines.

Documentos Relacionados

• Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis.
• Molecular Characterization of the Aeromonas hydrophila aroA Gene and Potential Use of an Auxotrophic aroA Mutant as a Live Attenuated Vaccine
• Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis.
• Molecular Cloning and Sequencing of the aroA Gene from Actinobacillus pleuropneumoniae and Its Use in a PCR Assay for Rapid Identification
• Molecular gene cloning and nucleotide sequencing and construction of an aroA mutant of Pasteurella haemolytica serotype A1.