Cloning and molecular characterization of the trithorax locus of Drosophila melanogaster.
AUTOR(ES)
Mozer, B A
RESUMO
The trithorax (trx) locus of Drosophila melanogaster affects segment determination primarily in the thoracic region. Mutant flies show transformations of the third and, to a lesser extent, first thoracic segment toward the second thoracic segment; abdominal transformations also occur. Prior genetic evidence suggested that these effects are based on interactions between trx and genes of the bithorax complex and Antennapedia complex. Further, interactions between the maternal effect locus female sterile homeotic (fsh) and trx have been observed. To aid in a molecular analysis of trx function, we have cloned the locus by a P-element transposon tagging approach. Five insertion mutations have been mapped within a region of about 10 kilobases; one of these mutations reverted coincident with the loss of the insertion. Transcription mapping suggests that two RNAs of about 12 and 15 kilobases are the major transcripts of the trx locus and that the transcription unit comprises a region of about 25 kilobases. Transcripts from the trx locus are distributed uniformly in early embryos, but at 14-16 hr after fertilization the ventral nerve cord contains a higher concentration of trx RNA than other regions of the embryo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=287215Documentos Relacionados
- Molecular cloning of Notch, a locus affecting neurogenesis in Drosophila melanogaster.
- Molecular cloning and genetic analysis of the suppressor-of-white-apricot locus from Drosophila melanogaster.
- Molecular cloning, germ-line transformation, and transcriptional analysis of the zeste locus of Drosophila melanogaster.
- Molecular characterization of bsg25D: a blastoderm-specific locus of Drosophila melanogaster.
- Molecular localization and developmental expression of the otu locus of Drosophila melanogaster.