Clonagem e expressão de β-ficocianina em amostras de Escherichia coli. / Cloning and expression of β-phycocyanin in Escherichia coli strains.

AUTOR(ES)

Tatiane Marques Porangaba de Oliveira

DATA DE PUBLICAÇÃO

2010

RESUMO

C-phycocyanin (C-PC) is a water soluble pigment and is present in Arthrospira platensis. It is consisted of two subunits, α and β, with molecular masses of 16 and 17 kDa, respectively. Recently it was shown that the β subunit of C-PC Anabaena has antitumor activity. The main objectives of this study were cloning and expression the β subunit of C-PC (β-PC) of A. platensis in Escherichia coli and check the ability of the C-PC and recombinant proteins in apoptotic induction in cancer (HEp-2) and non-cancer (fibroblasts) cells. The fibroblasts and HEp-2 cells were treated with 100 µg of recombinant and C-PC, proteins respectively for 6 h. After treatment, the cells were stained with toluidine blue (Critical Electrolyte Concentration Method - CEC). Apoptosis cells were detected by staining and morphological changes. The results show that the recombinant β-PC and C-PC proteins are able to induce apotosis in HEp-2 cells and dont induce it in fibroblasts cells.

ASSUNTO(S)

biotechnology biotecnologia apoptose gene expression cloning fibroblastos ficocianina escherichia coli clonagem expressão gênica arthrospira platensis escherichia coli apoptosis phycocyanin arthrospira platensis fibroblast

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