cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.

AUTOR(ES)

Kaye, J F

RESUMO

We have previously described a series of human immunodeficiency virus type 1-based vectors in which efficient RNA encapsidation appeared to correlate with the presence of a 1.1-kb env gene fragment encompassing the Rev-responsive element (RRE). In this report, we explore in detail the role of the RRE and flanking env sequences in vector expression and RNA encapsidation. The analysis of a new series of vectors containing deletions within the env fragment failed to identify a discrete packaging signal, although the loss of certain sequences reduced packaging efficiency three- to fourfold. Complete removal of the env fragment resulted in a 100-fold decrease in the vector transduction titer but did not abolish RNA encapsidation. We conclude that the RRE and 3' env sequences are not essential for human immunodeficiency virus type 1 vector encapsidation but may be important in vectors in which a heterologous gene has been placed adjacent to the 5' packaging signal, potentially disrupting its structure.

Documentos Relacionados

• Mutational analysis of cis-acting packaging signals in human immunodeficiency virus type 1 RNA.
• Packaging of human immunodeficiency virus type 1 RNA requires cis-acting sequences outside the 5' leader region.
• A conserved cis-acting sequence in the 5' leader of avian sarcoma virus RNA is required for packaging.
• cis-Acting Sequences Required for Coronavirus Infectious Bronchitis Virus Defective-RNA Replication and Packaging
• Analysis in human immunodeficiency virus type 1 vectors of cis-acting sequences that affect gene transfer into human lymphocytes.