A conserved cis-acting sequence in the 5' leader of avian sarcoma virus RNA is required for packaging.
AUTOR(ES)
Katz, R A
RESUMO
The deletion of a conserved sequence of ca. 30 nucleotides in the 5' noncoding leader region of an avian sarcoma virus DNA clone resulted in a loss of infectivity after transfection of chicken embryo fibroblasts. Genetic and biochemical analysis of a representative mutant demonstrated that the env gene was expressed normally. Thus, viral RNA transcription, splicing, and translation were not impaired. The amount of mutant viral RNA encapsidated into virions, however, was severely reduced despite the presence of helper-virus. We conclude that the deleted sequence is an essential cis-acting packaging signal.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=253052Documentos Relacionados
- Packaging of human immunodeficiency virus type 1 RNA requires cis-acting sequences outside the 5' leader region.
- cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.
- A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA.
- cis-Acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus.
- cis-Acting Sequences Required for Coronavirus Infectious Bronchitis Virus Defective-RNA Replication and Packaging