Characterization of proteolityc, hemorrhagic and edematogenic activities of BmHF-1 metallproteinase, isolet from Bothrops marajoensis crude venom / Caracterização das atividades proteolitica, hemorragica e edematogenica de uma metaloprotease BmHF-1 isolada a partir do veneno total de Bothrops marajoensis
AUTOR(ES)
Frank Denis Torres Huaco
DATA DE PUBLICAÇÃO
2009
RESUMO
In this present work we described the isolation, structural and functional characterization of a new metalloproteinase, named BmHF-1. This protein was isolated with a high degree of purity from the Bothrops marajoensis snake venom by the combination of two chromatographic steps, size exclusion in a Superdex G-75 column coupled to HPLC biocompatible system, followed by a HPLC-RP chromatographic procedure in a C-18 -Bondapack column. This new metalloproteinase had a molecular mass of 27162.36 Da determinate by MALDI-Tof mass spectrometry.....Note: The complete abstract is available with the full electronic digital thesis or dissertations
ASSUNTO(S)
bothrops marajoensis farmacologia bothrops marajoensis venom veneno - purificação metaloproteinas metalloproteins pharmacology
ACESSO AO ARTIGO
http://libdigi.unicamp.br/document/?code=000439595Documentos Relacionados
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