Characterization of a chromosomal gene and the antigen it expresses from the Lyme disease agent, Borrelia burgdorferi.
AUTOR(ES)
LeFebvre, R B
RESUMO
The sequence and characterization of a chromosomal gene from the Lyme disease agent Borrelia burgdorferi and the antigen it encodes are described. The gene was cloned and expressed in transformed Escherichia coli cells. The gene is composed of 597 bases and expresses a predicted protein of 199 amino acids. Antibodies specific for the recombinant antigen reacted with a single B. burgdorferi protein with a molecular mass of approximately 22 kDa. The protein was not susceptible to proteinase digestion but was extracted by n-butanol phase partitioning, suggesting a periplasmic location of the antigen. Sera from humans and canines seropositive for B. burgdorferi reacted with the recombinant antigen. The antigen characterized in this report appears to be immunologically significant in naturally infected hosts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265712Documentos Relacionados
- Further characterization of a potent immunogen and the chromosomal gene encoding it in the Lyme disease agent, Borrelia burgdorferi.
- Involvement of birds in the epidemiology of the Lyme disease agent Borrelia burgdorferi.
- Ticks and biting insects infected with the etiologic agent of Lyme disease, Borrelia burgdorferi.
- rRNA gene organization in the Lyme disease spirochete, Borrelia burgdorferi.
- Characterization of a circular plasmid from Borrelia burgdorferi, etiologic agent of Lyme disease.
