Characteristics of anaerobic oxalate-degrading enrichment cultures from the rumen.
AUTOR(ES)
Dawson, K A
RESUMO
Enrichment cultures of rumen bacteria degraded oxalate within 3 to 7 days in a medium containing 10% rumen fluid and an initial level of 45 mM sodium oxalate. This capability was maintained in serially transferred cultures. One mole of methane was produced per 3.8 mol of oxalate degraded. Molecular hydrogen and formate inhibited oxalate degradation but not methanogenesis; benzyl viologen and chloroform inhibited both oxalate degradation and methanogenesis. Attempts to isolate oxalate-degrading bacteria from these cultures were not successful. Oxalate degradation was uncoupled from methane production when enrichments were grown in continuous culture at dilution rates greater than or equal to 0.078 h-1. Growth of the uncoupled population (lacking methanogens) in batch culture was accompanied by degradation of 45 mM oxalate within 24 h and production of 0.93 mol of formate per mol of oxalate degraded. Oxalate degradation by the uncoupled population was not inhibited by molecular hydrogen or formate. Cell yields (grams [dry weight]) per mole of oxalate degraded by the primary enrichment and the uncoupled populations were 1.7 and 1.0, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=291668Documentos Relacionados
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