CD144, CD146 and VEGFR-2 properly identify circulating endothelial cell

AUTOR(ES)

Flores-Nascimento, Mariane Cristina, Alessio, Aline Morandi, Orsi, Fernanda Loureiro de Andrade, Annichino-Bizzacchi, Joyce Maria

FONTE

Rev. Bras. Hematol. Hemoter.

DATA DE PUBLICAÇÃO

2015-04

RESUMO

Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of con- sensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. Objective: The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells. Methods: Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies. Results: In spite of the rarity of the events, they were detectable and presented similar interperson numbers of circulating endothelial cells. Conclusion: The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.

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