CD144, CD146 and VEGFR-2 properly identify circulating endothelial cell
AUTOR(ES)
Flores-Nascimento, Mariane Cristina, Alessio, Aline Morandi, Orsi, Fernanda Loureiro de Andrade, Annichino-Bizzacchi, Joyce Maria
FONTE
Rev. Bras. Hematol. Hemoter.
DATA DE PUBLICAÇÃO
2015-04
RESUMO
Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of con- sensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. Objective: The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells. Methods: Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies. Results: In spite of the rarity of the events, they were detectable and presented similar interperson numbers of circulating endothelial cells. Conclusion: The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.
Documentos Relacionados
- Autocrine stimulation of VEGFR-2 activates human leukemic cell growth and migration
- Expressão do fator de crescimento endotelial vascular (VEGF) e seus receptores VEGFR-1 e VEGFR-2 durante o início da gestação em camundongos.
- Vascular atrophy and VEGFR-2 signaling: old theories of pulmonary emphysema meet new data
- Perfil imunoistoquimico dos receptores VEGFR-1 e VEGFR-2 em tres fases do desenvolvimento pulmonar fetal no modelo de hernia diafragmatica congenita induzida pelo nitrofen
- Expressão do VEGFR-2 em polpas de dentes decíduos e permanentes jovens humanos