Catechol-O-methyltransferase and TMD in women / Polimorfismo genetico da Catecolamina-0-Metiltransferase (Val 158Met) e disfunção temporomandibular em mulheres

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

Pain is a complex experience encompassing behavioral, environmental, and genetics factors. Temporomandibular Disorders (TMD) are a musculoskeletal condition considered to have multifactorial etiology comprising local, systemic and genetic factors, which makes individuals respond differently to the development and progression of the disease. Thus, intrinsic genetic conditions can be related to pain sensitivity and risk of developing TMD. Catechol-O-methyltransferase (COMT) is an enzyme involved in the metabolic degradation of endogenous catecholamines and whose activity influences modulatation of some pain sensitivity neurotransmitters. A single nucleotide polymorphism (SNP: rs4680) in codon 158 (Val158Met) of the gene encoding COMT may be related to pain sensitivity and risk of developing TMD. The purpose of the present study was to investigate the association among COMT polymorphism (Val 158 Met) and TMJ pain sensitivity in women with TMJ disorders. TMD diagnosis was obtained using the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) and this blinded study was designed as follows: 1) Control group: 334 volunteers with no TMD signs and symptoms 2) TMD Group with no pain : 224 volunteers with TMD signs but no TMJ pain and 3) TMD Group with pain: 75 volunteers with TMD signs and TMJ pain. Deoxyribonucleic acid (DNA) from all volunteers was obtained from buccal mucosa cells. COMT gene was amplified by polymerase chain reaction (PCR). Alleles were detected by restriction fragment length polymorphism (RFLP), followed by electrophoresis on a 10% polyacrylamide gel. Hardy-Weinberg equilibrium was tested to determine the balance in alleles distribution in the population studied. Differences in genotype frequencies among control group, TMD group with no pain and TMD Group with pain were assessed by chi (2) test, with significance level set at 5%. No statistically significant associations were found between COMT (Val158Met) polymorphism and pain sensitivity in TMJ, suggesting that this polymorphism can not be related to high pain sensitivity in TMJ.

ASSUNTO(S)

genotype temporomandibular joint genotipo dor pain articulação temporomandibular

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