Caracterização molecular e avaliação da resistência ao vírus da tristeza dos citros (CTV) em plantas transgênicas de laranja Valência(Citrus sinensis L. Osbeck) / Molecular characterization and resistance evaluation to citrus tristeza virus (CTV) in transgenic plants of Valência orange (Citrus sinensis L. Osbeck)

AUTOR(ES)

Fabiana Rezende Muniz

DATA DE PUBLICAÇÃO

2009

RESUMO

In Brazil, citrus is one of the most important cultures. The productivity of this culture in the country is still considered low and this fact is due to several pests and diseases that affect the crop. Among the diseases there is the tristeza, caused by Citrus tristeza virus (CTV). This pathogen can also be related with another important disease, the citrus sudden death. Therefore, CTV acquired much more significance. This work aimed to characterize with molecular analysis and to evaluate the resistance to CTV of transgenic Valência plants (Citrus sinensis L. Osbeck), containing genomic fragments of CTV, in three different transgenic constructs. The plants were confirmed as transgenic by Southern blot. The transcription of the transgene was evaluated by RT-PCR. The transgenic plants were challenged with a weak strain of CTV, CTV-IAC, by bud inoculation with two infected bubbles, and by the infected vector Toxoptera citricida. After four weeks of inoculation, the evaluation of viral replication in the transgenic seious was done by ELISA indirect sandwich with monoclonal antibody against the CTV coat protein. The results indicated variation of the resistance to the translocation of the virus between the different transgenic constructs used and between clones of the same plant. All the inoculated plants indicated the presence of the virus in, at least, one of the three evaluated clones, when inoculated by grafting. When inoculated by the vector some plants had all their clones with low values of virus, indicating a possible resistance to the pathogen.

ASSUNTO(S)

frutas cítricas genética molecular vegetal tristeza cítrica. elisa plant genetic resistance transgenic plants citric fruits citrus tristeza. elisa plantas transgênicas plant molecular genetics resistência genética vegetal

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