Caracterização de um novo begomovírus, Euphorbia yellow mosaic virus, no Brasil / Characterization of Brazilian begomovirus Euphorbia yellow mosaic virus

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The amendoim-bravo (Euphorbia heterophylla; also known as painted euphorbia) is an important weed in several countries, interferes in the production of cultivated plants, and has a great potential as a reservoir of plant viruses. The objective of this study was to study the diversity of begomoviruses that infect amendoim-bravo plants, and characterize molecular and biologically one isolate enabling its complete identification. Seven samples were collected of amendoim-bravo showing yellow mosaic at distinct localities in Goiás State and Federal District/Brazil. They were tested by PCR using universal begomovirus primers and it was confirmed that all seven samples were positively infected. These DNA samples were used as template for rolling circle amplification (RCA) using the enzyme phi-29 DNA polymerase. Each amplified product was digested with a single-cutting restriction enzyme and cloned into pBluescript vector (Statagene). The complete sequence of both components (eight DNA-A clones and three DNA-B clones) were obtained using internal and vector primers. For the biological characterization, infectious clones were prepared and inoculated by particle bombardment to cultivated plants and weeds. The infection was confirmed by PCR in plants of Capsicum annuum, Datura stramonium, Nicotiana benthamiana and Euphorbia heterophylla. All eight DNA-A sequences shared 86,9% nucleotide identity with Euphorbia mosaic virus Peru (accession AM886131, the most closely related begomovirus sequence), while DNA-B shared 61,6% nucleotide identity with Tomato commom mosaic virus (accession NC_010836). According to the International Committee on Taxonomy of Viruses (ICTV) a virus is considered as a new species when the sequence identity with another virus is less than 89%, suggesting that it is distinct from Euphorbia mosaic virus Peru. Therefore, according to the criteria established by the ICTV, the viruses characterized in this work can be considered as a new species within the genus Begomovirus, and the name Euphorbia yellow mosaic virus is proposed.

ASSUNTO(S)

begomovírus euphorbia yellow mosaic virus euphorbia heterophylla virologia vegetal ciencias biologicas

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