Capacidade de aderÃncia Ãs cÃlulas epiteliais da cavidade oral e perfil histoquÃmico de culturas de candida estocadas na micoteca urm / Adherence capacity to epithelial cells and histochemistry profile of candida spp. stocked in urm culture collection

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Several studies report the adherence capacity of Candida spp. to epithelial cells, but comparative studies based in this pathogenicity factor versus cellular recognition via cell surface carbohydrates are scarce. This study aimed to evaluate the adherence ability of 20 Candida albicans, six C. krusei, 15 C. parapsilosis and five C. tropicalis strains to human buccal epithelial cells according the expression of cell surface carbohydrates evaluated via lectin histochemistry. Adherence assays were carried out incubating epithelial cells with the yeast suspensions under stirring. Lectin histochemistry was examined using peroxidase conjugated lectins. Results showed that C. albicans and C. tropicalis were more adherents than C. krusei and C. parapsilosis (P<0.01), besides to present high _-L-fucose moieties in the cell surface. Three isolates of C. parapsilosis presented similar adherence values and histochemistry profile to that of C. albicans and C. tropicalis (r=0.8336, P=0.0001). All C. krusei strains adhered weakly, showing the low pathogenicity inherent to this species. Moreover, our results showed the presence of _-D-glucose/_-D-mannose, N-acetyl-Dglucosamine/ N-acetylneuraminic acid and D-galactose/N-acetyl-D-galactosamine in fungal cell wall. Complex carbohydrates on the surface of Candida species may represent additional motifs through which interactions of this fungus with host cells could be established

ASSUNTO(S)

micologia lectinas epithelial cells adherence candida lectin histochemistry aderÃncia cÃlulas epiteliais candida histoquÃmica

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