cap20 gene pathogenicity in Colletotrichum gloeosporioides of the isolates / Gene de patogenicidade cap20 em isolados de Colletotrichum gloeosporioides

AUTOR(ES)

Danielli Barreto Maciel

DATA DE PUBLICAÇÃO

2008

RESUMO

The early event for the penetration in some Colletotrichum species, iniciates with conidium adesion and germination on the surface of the host plant, producing germinative tube and then forming the apressorium, wich penetrates directly in the cuticle. Previous studies have identified in Colletotrichum gloeosporioides genes called cap, wich are only expressed during the apressorium formation after 4 hours of conidium exposition to waxes present on the cuticle of avocado (Persea gratissima). The objective of this work was to amplify the pathogenicity cap20 gene and to analyze the variation of this gene in isolates of C. gloeosporioides from different hosts. The genetic variability was accessed using RFLP-ITS and Intron (to primer EI1) markers and grouping by UPGMA method. Primers for cap20 gene were constructed from selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) using the Primer 3 program. The analysis of the dendrograms showed that the RFLP-ITS marker was able to separate the species C. gloeosporioides, C. acutatum and C. sublineolum. On the other hand, these results also showed that the primer EI1 of Intron Splice Site evidenced greater genetic variability than the RFLP-ITS, however did not separate the species. The constructed primers named P1 and P2 for cap20 gene had been efficient to amplify C. gloeosporioides (except for five mango isolates and one chili isolate), C boninense, C. dematium, C. capsici and C.gossypii var. cephalosporioides, but did not amplifyd C. graminicola, C. acutatum, C. sublineolum and C. coccodes, suggesting that this gene can present structure differences among C. gloeosporioides isolates related to the host and that this gene is also present in the other cited species of Colletotrichum. The digestion with MspI enzime for isolates of the C. gloeosporioides that presented amplification did not evidence cap20 gene structure differences

ASSUNTO(S)

colletotrichum gloeosporioides micologia gene cap20 patogenicidade cap20 gene pathogenicity colletotrichum gloeosporioides

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