Variability of isolates of Colletotrichum gloeosporioides, upon the pathogenicity to mango fruits (Mangifera indica l.), rapd markers and region its-rdna. / Variabilidade de isolados de Colletotrichum gloeosporioides, quanto a patogenicidade a frutos de mangueira (Mangifera indica l.), marcadores rapd e regiÃo its do rdna.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

The anthracnose caused by the fungus Colletotrichum gloeosporioides is one of the most serious mango diseases, being a limiting factor for the production of healthy and commercializable fruits. The present work had the aim to analyze the variability of C. gloeosporioides isolates, upon the pathogenicity on mango fruits, RAPD markers and region ITS-rDNA. Five isolates obtained from onion and ten from mango at different areas of Pernambuco State were used. The pathogenicity test was carried out with fruits of the varieties Rosa and Espada. For the RAPD analysis were used the OPX-15, OPX-02, OPX-06, OPA-11 and OPA-02 primers and for the rDNA locus ITS-5.8S-ITS2 amplification, the ITS1 and ITS4 primers. The isolates proceded from mango with anthracnose symptoms were more aggressive to both studied varieties than the isolates from onion. Through the cluster generated by the RAPD analysis, two groups were lined, separating all isolates of mango from the analysis of amplification products of the region ITS-5.8S-ITS2-rDNA, however, the product digestion with the enzyme MspI showed difference to the isolate URM 4596 (Mangifera indica L./Ilha de ItamaracÃ-PE)

ASSUNTO(S)

colletotrichum gloeosporioides rapd regiÃo its do rdna colletotrichum gloeosporioides micologia rapd region its-rdna

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