Blue light-induced Absorbance Changes in Membrane Fractions from Corn and Neurospora12
AUTOR(ES)
Brain, Robert D.
RESUMO
Blue light-induced absorbance changes were measured from differentially centrifuged membrane fractions from dark-grown coleoptiles of Zea mays L., and mycelia from an albino mutant of Neurospora crassa. Actinic irradiation caused changes in absorbance consistent with a flavinmediated reduction of a b-type cytochrome. Both corn and Neurospora showed similar light-minus-dark difference spectra, dose response curves, and kinetics of dark recovery after irradiation. The photoreducible cytochrome system from Neurospora showed the same distribution as the activity of a sodium-stimulated adenosine triphosphatase, thought to be a plasma membrane marker, in differential centrifugation experiments. The fraction showing the absorbance change did not co-sediment with the mitochondria, nor with the endoplasmic reticulum. Comparison of absorption spectra of fully oxidized, partially reduced, and fully reduced preparations showed that approximately a 30% reduction of the cytochromes involved with the process was needed to obtain the light-induced absorbance changes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=543332Documentos Relacionados
- Fractionation of Chlorophyll Forms from Euglena and Measurement of Light-Induced Absorbance Changes 12
- Light-Induced Absorbance Changes Associated with Phototaxis in Dictyostelium
- Light-induced Changes in Allophycocyanin 1
- Light-induced reorientation in the purple membrane.
- Blue Light-Induced Phosphorylation of a Plasma Membrane-Associated Protein in Zea mays L.
