Biochemical and biological characterization of toxins in the venom and mucus of the catfish Cathorops spixii. / Caracterização bioquímica e biológica de toxinas presentes na peçonha e no muco do bagre Cathorops spixii.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Of the venomous fish found in Brazil, catfish noteworthy for the number of accidents they cause. The objective of this study was to characterize the toxic compounds present in the venom and mucus. We obtained an average protein intake of 3,1 mg / mL (venom) and 1,4 mg / mL (mucus). The electrophoretic profile of venom and mucus has a few protein bands. Fractionation isolated 11 fractions to the venom and 13 fractions to the mucus. With respect to biological activities evaluated, the peptide fractions induced tissue damage while the protein fractions induced inflammation. We isolated two fractions with antimicrobial activity for each sample. We isolated a toxin of 65,1 kDa which shows homology with Wap65 and intravital microscopy analysis revealed that this protein causes an increase in leukocyte rolling as well as the presence of leukocytes adhered to the endothelium. Our results indicate a difference between the peptide and protein components of mucus and venom. Finally, we isolate and characterize the protein Wap65 from the venom of this fish.

ASSUNTO(S)

biotecnologia wap 65 toxinas bagre marinho marine catfish venenos wap65 toxins muco nenomous fishes peixes venenosos biotechnology venoms mucus

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