Avaliação do papel da conexina 43 na angiogênese, experimentalmente induzida em córnea de camundongos / Evaluation the role of connexin 43 during angiogenesis, experimentally induced in mice córnea

AUTOR(ES)

Lucas Campos de Sá Rodrigues

DATA DE PUBLICAÇÃO

2005

RESUMO

The GAP junctions are intercellular streams responsible for the communication between close cells, which allow small molecules and ions to pass through them maintaining the cellular homeostasis. The GAP junction is formed of six proteins, the connexin. In the endothelial cell, there are the connexin 37, 40 and 43. In this study, we stimulated the angiogenesis in the mices cornea through its cauterization using silvers crystal glass. It was used heterozygote mice to the gene of connexin 43 (Cx43+/-) and wild mice (Cx43+/+). The corneas were analyzed 2 and 6 days after the cauterization through the vascular morphology, detection of Cx37, Cx40, Cx43, PCNA through Western Blot and ultrastructural evaluation of the endothelial cells. As a result, we obtained a smaller area of vascular fillness in the animals Cx43+/- with 2 and 6 days of corneal injury, however, in regard to the extensions of the vessels, it wasnt observed any changes between the groups. A smaller proliferation of cells was verified, through the detection of PCNA, in the heterozygote animals only 2 days after the corneal injury. There wasnt any modification of the Cx37 and Cx40 between groups. The Cx43 seems to be an important connexin to the endothelial cell during the process of angiogenesis.

ASSUNTO(S)

angiogênese connexin 43 angiogenesis córnea camundongos cornea mice conexina 43

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