Avaliação do efeito radioprotetor do selenito de sodio na reparação de tibias de ratas ovariectomizadas / Evaluation of the radioprottector effect of sodium selenite on bone healing in the tibia of ovariectomized rats

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Osteoporosis and ionizing radiation affect the bone healing and people can suffer both conditions, especially older women. At the moment, antioxidant radioprotectors have been evaluated, such selenium compounds. This study aimed at evaluating the selenium protection in the bone repair process in ovariectomized rats submitted to an irradiation procedure. For this purpose, eighty ovariectomized female Wistar rats were randomly divided in four experimental groups: ovariectomized, ovariectomized/selenium, ovariectomizedlirradiated and ovariectomized/selenium/irradiated. Abone defect was made on all animals tibias forty days after ovariectomy. Two days after surgery, only ovariectomized/selenium and ovariectomized/seleniumlirradiated rats received 0.8 mg Se/Kg. Three days after surgery, only ovariectomized irradiated and ovariectomized/selenium/irradiated rats received 10 Gy of X rays on the lower limbs region. The animals were sacrificed 7, 14, 21 and 28 days after surgery in order to assess the repair process, which was evaluated by morphologic analysis in Masson Tricromic. lt was also evaluated by analysis of trabecular bone number in Masson Tricromic and birefringence analysis in Picrosirius. It was possible to observe a delay in the bone repair process in the irradiated/ovariectomized group and similarity between ovariectomized, ovariectomized/selenium and ovariectomized/selenium/irradiated, which proved the selenium radioprotection without its toxicity

ASSUNTO(S)

radioterapia radiotherapy osteoporose osteoporosis

ACESSO AO ARTIGO

Documentos Relacionados