Avaliação da comunidade microbiana procarionte através de técnicas moleculares - FISH, PCR/DGGE e sequenciamento em sistemas artificiais de redução de cargas: ênfase ao estudo de lagoa de estabilização facultativa. / Prokariotic microbial community assessment by molecular techniques - FISH, PCR/DGGE and sequencing in load reduction artificial systems - enphasys on the study of facultative stabilization pond.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

Microorganisms are amongst the most responsible for the conversion of sewage organic compounds and they are the key of the treatment success. The combination of two or more molecular techniques in this kind of assessment allows getting more accurate results concerning a microbial community. The facultative stabilization pond microbial community structure of domestic sewage treatment from Cajati city was characterized based on 16S RNAr gene fragments patterns from PCR-DGGE, FISH, 16S RNAr gene clone library and sequencing. The patterns obtained by DGGE were co-related to environmental variables by redundancy analysis (RDA). The samples were collected in three intervals to study the seasonal variation, it was collected in three depth (surface, 0,7 m and 1,5 m) to the vertical assessment and in nine spots at 0,7 m depth for the horizontal assessment of the microbial community.

ASSUNTO(S)

comunidade microbiana dgge dgge fish fish lagoa de estabilização microbial community polymerase chain reaction reação em cadeia por polimerase sequenciamento sequencing stabilization pond

ACESSO AO ARTIGO

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