Avaliação da atividade de eosinofilos perifericos em pacientes com doenças inflamatorias intestinais

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Eosinophils are involved in the pathogenesis of several chronic inflammatory diseases, including inflammatory bowel disease (ffiD), as Crohn s disease (CD) and ulcerative colites (DC). However, how activation of intravascular eosinophils contributes to disease activity or ffiD pathogenesis is still unknown. The aim of this study was to investigate if eosinophils isolated ITomperipheral blood ITomffiD patients are activated compared to healthy volunteers. Eosinophils ITomhea1thyvolunteers (n=5), CD (n=5) and DC patients (n=5) were purified using a Percoll gradient followed by immunomagnetic cell separator. Eosinophil activity was investigated using chemotaxis, adhesion and mediator release assays (eosinophil peroxidase, EPO), flow cytometric, histologic counting and morphometric analysis. -formyl-metionyl-Ieucyl-fenilanina (fMLP) induced eosinophil chemotaxis was significandy augmented in cells isolated from CD and DC patients compared to hea1thyvolunteers. Eosinophil basal adhesion of CD significantlyincreased to fibronectin or serum if compared to DC or hea1thyvolunteers. Basal and stimulated EPO release was reduced in cells ITomCD patients compared to hea1thyvolunteers. However, basal EPO release was significantly enhanced in cells isolated from DC patients when compared to hea1thy volunteers. The expression of adhesion molecules macrophage-l antigen (Mac-l) and very late antigen 4 (VLA-4) isn t different in patients with DC and CD compared to hea1thyvolunteers. Histologic counting of eosinophils in intestinal tissue and morphometric analysis of granules of this cells showed, respectively, significant augmentation in the number of eosinophils and its granules in CD and DC patients compared to hea1thy volunteers. Our results suggest that peripheral eosinophils are preactivated in ffiD patients. Activation of peripheral eosinophils followed by migration to intestinal tissue probably contributes to ffiD pathogenesis

ASSUNTO(S)

adesão microscopia eletronica quimiotoxia ulcerative colite crohn s disease inflammatory bowel disease eosiphils

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