Avaliação da associação entre o polimorfismo dos genes IL-1A (-889), IL-1B (-511), IL-1B (+3954), IL-1RN (íntron2-VNTR) e TNFA (-308) e a periodontite agressiva / Correlation between IL-1A (-889), IL-1B (-511), IL-1B (+3954), IL-1RN (intron 2-VNTR) and TNFA (-308) gene polymorphisms and aggressive periodontitis

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Aggressive periodontitis (AgP) is a type of periodontal disease characterized by a rapid destruction of the periodontal supporting tissues in young adults who are usually systemically well. Population and family studies indicate that genetic factors seem to have a strong influence on susceptibility to AgP. Genetic polymorphism at the interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFA) were associated with the increase on the severity of periodontitis. This study aimed at finding a possible association between IL-1A (-889), IL-1B (-511), IL-1B (+3954), IL-1RN (intron 2-VNTR) and TNFA (-308) polymorphism in patients with aggressive periodontitis. Genomic DNA was extracted from the saliva of 150 nonsmoking subjects, 50 patients with AgP and 100 periodontal healthy subjects. All individuals were systemically healthy. The polymorphisms of IL-1A (-889), IL-1B (-511), IL-1B (+3954) and TNFA (-308) were analyzed by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The polymorphism of a variable number tandem repeat (VNTR) in intron 2 of the IL-1RN gene was detected by PCR amplification and fragment size analysis. Statistical analysis revealed no significant difference in the IL-1A (-889), IL-1B (-511), IL-1B (+3954) and TNFA (-308) genotypes and allele frequencies between AgP patients and periodontal healthy subjects (p-value>0.05%). However, there were significant differences among the groups in the distribution of IL-1RN (íntron 2-VNTR) genotypes (Fishers exact test; p-value=0.021) and allele frequencies (Fishers exact test; p-value=0.04). These findings suggest that IL-1RN (intron 2-VNTR) polymorphism is associated with AgP in the population presented here.

ASSUNTO(S)

aggressive periodontitis periodontite agressiva interleukin-1 periodontal disease interleucina-1 polymorphism fator de necrose tumoral-alfa polimorfismo tumor necrosis factor-alpha doença periodontal

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