Avaliação comparativa de cascas e folhas de Ziziphus joazeiro Mart (RHAMNACEAE) em relação aos perfis fitoquimico e toxicológico e as atividades antioxidante e antimicrobiana / Comparative evaluation of barks and leaves Ziziphus joazeiro Mart. (Rhamnaceae) in relation to the profiles phytochemistry and toxicological and antioxidant and antimicrobial activities

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The presence of free radicals have been correlated with a great number of illnesses, such as cancer, renais, hepáticas, pulmonary, intestinais, cerebral illnesses, to articulate and oftamológicas. The methodologies most common to determine the antirust activity in practical way, fast sensible e are the ones that involve a radical cromóforo, simulating the reactive species of oxygen (EROs), being free radical DPPH (1,1-difenil-2-picrilhidrazil) one of the most used. The phenolic composites gifts in vegetables have received considerable attention for being the main components with antirust activity, that play important role in the neutralization of free radicals. The objective of this study was comparativily to evaluate the barks and leaves of Ziziphus joazeiro Mart. (Joazeiro) with regard to the antirust activity, the tannins and flavonoids, the fitoquímico profile and toxicology preliminary front the Artemia salina Leach. and antimicrobial activity. The collections had been carried through in the city of Altinho - PE. The fitoquímica selection was carried through by chromatography in thin layer, the quantitative tannins analysis and flavonoids was based on the technique of complex for Folin-Ciocalteu and AlCl3, respectively, the antirust action was analyzed by the capacity of antirust substances gifts in the samples will catch free radical DPPH. The toxicology profile of extracts was evaluated front Artemia saline Leach. e the based antimicrobial activity in the described methodology for Bauer-Kirby. The data of these quantifications had received factorial delineation and statistical treatment through Software BioEstat 4.0. One observed differences in texts of total phenols, tannins and flavonoids for the two studied parts (barks and leaves). The leaves of Z. joazeiro had more presented phytochemistry diversity accented that the barks, however, glico-coumarins had not been visualized in leaves and yes only in the barks. The test with larvae of A. saline indicated that extracts of barks (LC50 = 796.6 μg /mL) and leaves (LC50 = 609.46 μg /mL) are moderately toxic. With regard to antioxidant activity, the leaves showed better performance (IC50 = 461.8816 μg /mL), being more efficient than the bark (IC50 = 1743.0541 μg /mL). The species was strongly active against 66% of the bacteria tested. The extract of the leaves showed MIC of 0.25-0.5 mg/mL against Micrococcus luteus (UFPEDA 100) and 0.125-0.250 mg/mL against Mycobacterium smegmatis (UFPEDA 71), the extract of the bark showed MIC 0.5-1.0 mg/mL against Mycobacterium smegmatis (UFPEDA 71). The levels of metabolites present in leaves are higher than those present in the bark, so the barks (the popularly used) can be replaced by leaves, so as to prevent the decimation of the species. We observed the presence of secondary metabolites in the studied species not mentioned in the literature. Both extracts were moderately toxic. The herbal extract of Z. joazeiro due to its antimicrobial activity can be considered a promising for the treatment of diseases caused by bacteria.

ASSUNTO(S)

farmacia artemia salina, antimicrobial ziziphus joazeiro joazeiro artemia salina antioxidant ziziphus joazeiro antioxidante antimicrobiano caatinga caatinga

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