AvaliaÃÃo da reaÃÃo em cadeia da polimerase (PCR) e mÃtodos convencionais no diagnÃstico da micobacteriose extrapulmonar exceto renal. / Evaluation of polymerase chain reaction (PCR) and conventional methods for diagnosis of extrapulmonary mycobacteriosis except renal

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

28/02/2005

RESUMO

In order to focus on the importance of laboratorial diagnosis of extrapulmonary mycobacteria, except renal, we employed the polymerase chain reaction (PCR). Clinical samples (body fluids) were tested by PCR for the presence of Mycobacterium tuberculosis complex (MTB) and nontuberculous mycobacteria (MNTB). We compared the results obtained through the PCR with the ones obtained through conventional microbiological methods. The 114 clinical samples were obtained from internal patients or from or in ambulatorial attendance with clinical suspicion of extrapulmonary tuberculosis, except renal. The samples were collected from 2001 to 2003, and ceded to this research by the LaboratÃrio de Microbiologia do LaboratÃrio Central do Hospital UniversitÃrio Walter CantÃdio (HUWC-UFC), and by the LaboratÃrio Central de SaÃde PÃblica da Secretaria de SaÃde do Estado do Cearà (LACEN-CE). The direct microscopy and culture were negative in all the samples. The molecular research, for the Mycobacterium genus was positive in 10 samples, and for the Mycobacterium tuberculosis complex was negative in all the samples.

ASSUNTO(S)

mycobacterium tuberculosis pcr nontuberculous mycobacteria extrapulmonary tuberculosis anatomia patologica e patologia clinica mycobacterium tuberculosis pcr micobactÃrias nÃo pertencentes ao complexo mycobacterium tuberculosis tuberculose extrapulmonar

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