Atividades biológicas de polissacarídeos sulfatados extraídos da alga vermelha gracilaria caudata

AUTOR(ES)

Leandro Silva Costa

DATA DE PUBLICAÇÃO

2008

RESUMO

In recent years, sulfated polysaccharides (SP) from marine algae have emerged as an important class of natural biopolymers with potential pharmacology applications. Among these, SP isolated from the cell walls of red algae have been study due to their anticoagulant,antithrombotic and anti-inflammatory activities. In the present study, three sulfated polysaccharides fractions denominated F1.5v, F2.0v and F3.0v were obtained from seaweed G. caudate by proteolysis followed to acetone fractionation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9,0, stained with 0.1% toluidine blue, showed the presence of SP in all fractions. The chemical analysis demonstrated that all the fractions are composed mainly of galactose. These compounds were evaluated in anticoagulant, antioxidant and antiproliferative activities. In anticoagulant activity evaluated through aPTT and PT tests, no one fractions presented anticoagulant activity at tested concentrations (0.1 mg/mL; 1.0 mg/mL; 2.0 mg/mL).The antioxidant activities of the three fractions were evaluated by the following in vitro systems: Total antioxidant capacity, superoxide and hydroxyl radical scavenging, ferrous chelating activity and reducing power. The fractions were found to have different levels of antioxidant activity in the systems tested. F1.5v shows the highest activity, especially in the ferrous chelating system, with 70% of ferrous inhibiting at 1.0 mg.mL-1. Finally, all the fractions showed dose-dependent antiproliferative activity against HeLa cells. The fractions F1.5v and F2.0v presented the highest antiproliferative activity at 2.0 mg/mL with 42.7% and 37.0% of inhibition, respectively. Ours results suggests that the sulfated polysaccharides from seaweed G. caudata are promising compounds in antioxidant and/or antitumor therapy

ASSUNTO(S)

antioxidant red algae gracilaria caudata radicais livres free radicals atividade anticoagulante bioquimica atividade antiproliferativa gracilaria caudata anticoagulant atividade antioxidante antiproliferative

Documentos Relacionados

• Immunostimulant effect of sulfated polysaccharides from the red marine alga Gracilaria caudata in sexual reversion of Nile tilapia, Oreochromis niloticus (LINNAEUS, 1766) under adverse conditions.
• Atividades antinociceptiva e antiinflamatÃria dos polissacarÃdeos sulfatados da alga marinha vermelha Gracilaria cornea J. Agardh
• CaracterizaÃÃo estrutural e propriedades antitumorais dos polissacarÃdeos extraÃdos da alga marinha vermelha Gracilaria caudata J Agardh
• Caracterização parcial e atividades farmacológicas do extrato rico em polissacarídeos sulfatados da angiosperma marinha Halodule wrightii
• Avaliação do efeito de polissacarídeos sulfatados da macroalga Gracilaria birdiae sobre as condições de imunocompetência de camarões Litopenaeus vannamei infectados com o vírus da síndrome da mancha branca (WSSV)