Atividade hipoglicemiante da trans-desidrocrotomia estraÃda do Croton Cajucara encapsulada em microesferas de copolÃmero de Ãcido lÃctico e glicÃlico

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

This work aims the achievement of PLGA microparticles containing DCTN, their physicochemical characterization as well as the evaluation of their in vitro kinetics profile and in vivo hypoglicemic activity. The PLGA microspheres were obtained by a multiple emulsion technique followed by solvent evaporation. The microspheres morphology were evaluated by light microscopy and electron scanning microscopy. The DCTN into microspheres was analyzed by UV spectrophotometry. The in vitro kinectics was accomplished using phosphate buffered solution (pH 7.4), simulating physiologic conditions. At pre-determined time intervals, samples were collected, filtered and stored for later analysis of DCTN content. The in vivo evaluation was performed in both normal and diabetic Swiss mice. Diabetes was induced with streptozotocin and the DCTN (50 mg.Kg-1) was orally administered after fourteen days during 7 days. The glicemia levels were daily evaluated. The results showed that the DCTN encapsulation efficiency was of 85.46 Â 3.37 %. Microspheres presented a smooth spherical surface with a mean diameter of 3.11 Â 0.56 μm. The in vitro kinetics demonstrated a profile of gradual liberation that reached approximately 60 % at the third day. DCTN-loaded microspheres not only showed better biodisponibility, but were also capable to decrease the glicemia in normal mice. However, this effect was not observed for diabetic mice submitted to a one week treatment. Based on these findings, it can be concluded that microspheres containing DCTN have potential use in the control of some hyperglicemic states

ASSUNTO(S)

trans-desidrocrotonina micropartÃculas sistema de liberaÃÃo controlada dctn trans-dehydrocrotonin farmacia dctn drug delivery system microparticles microesferas microspheres

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