Atividade antimicrobiana do óleo essencial de diferentes acessos de Mentha spp. contra Candida albicans e Candida dubliniensis / Antimicrobial activity of essential oils from different accessions of Mentha spp. against Candida albicans and Candida dubliniensis

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The aim of this study was to evaluate the antimicrobial activity of essential oils and fractions of different accessions of Mentha spp. against Candida spp., identifying compounds and verifying its cytotoxicity.The essential oils of 64 different accessions of Mentha spp. were extracted using the fresh aerial parts of the plant by hydrodistillation cleavenger type system Then, The the oils were tested against clinical isolates and reference strains Candida albicans (CBS 562) and Candida dubliniensis (CBS 7987). The minimal inhibitory concentration (MIC) was determined by microdilution method, using the CLSI protocol. The best accessions tested on planktonic cells were separated into 5 parts by dry column, and oils and fractions were subsequently tested against biofilms of C. albicans (SC 5314). With standardized inoculum in RPMI-1640, the biofilms were produced in sterile polyethylene plates with 96 wells, for to evaluate the oils and fractions in preformed biofilm and inhibition biofilm formation. After the biofilm was quantified with XTT solution and analyzed by optical and confocal microscopy. The compounds were further identified by thin layer chromatography (TLC) and gas chromatography mass spectrometry (GC-MS). The cytotoxicity was also tested in epithelial cells as well as antitumor activity, by evaluating the antiproliferative activity in different cell lines using the test sulforrodamina B (SBR) for evaluation of cell growth.The best yield in essential oil (dry basis) was observed for the accessions M. rotundifolia CM 31 (1.5% dry basis). Among the 64 oils, four stood out, showed strong activity with broad spectrum, inhibiting all strains tested, and these, M. canadensis (CM 05) - (<0.007 to 0.187 mg/mLto 0.500 mg/ml), M. spicata (CM 30) - (0.062 mg/mL to 0.500 mg/ml), M. arvensis CM 36 (<0.007 mg/mL to 1 mg/mL) and M. spicata x suaveolens (CM 52) - (0.062 mg/mL to 0.500 mg/mL). Fractions F2, F3 M. spicata x suaveolens CM 52 showed the best activity among the tested oils and fractions, showing 49% and 38% inhibition at 0.5 mg/mL for preformed biofilm and 70% inhibition (at 0.062 mg/mL) and 61% inhibition (at 0.125 mg/mL), respectively, for inhibitory biofilm formation. These results were confirmed by microscopic analysis. The mainly oil M. canadensis 05 CM were carvone (74.84%), linalool (4.84 %); M. spicata CM piperitenone oxide were 30 (28.05%), beta-E-farnesene (18.58%) and gamma-muuroleno (17.38%); M. arvensis CM 36 were Linalool (33.24%), acetate, linalool (21.97%) and alphaterpineol (11.77%); M. spicata x suaveolens CM 52 were pulegone (52.20 %), piperitenone (29.51 %) and gamma-muurolene (4.44 %). Fractions F2 and F3 showed antiproliferative activity for most strains, showing lack of cytotoxicity in epithelial cells tested.The essential oils of Mentha spp. exhibit antimicrobial activity against planktonic cells of Candida spp. (moderate to weak), except oils originating from accessions of M. canadensis CM 05, M. spicata CM 30, M. arvensis CM 36 and M. suaveolens x spicata CM 52. The fractions F2 and F3 of Mentha spicata x suaveolens CM 52 shows antimicrobial effect on preformed biofilm and inhibition biofilm, and absence of cytotoxicity to mammalian cells

ASSUNTO(S)

plantas medicinais mentha spp candida spp biofilme citotoxicidade medicinal plants mentha spp candida spp biofilms cytotoxicity

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