AtCSLA7, a Cellulose Synthase-Like Putative Glycosyltransferase, Is Important for Pollen Tube Growth and Embryogenesis in Arabidopsis1

AUTOR(ES)

Goubet, Florence

FONTE

American Society of Plant Biologists

RESUMO

The cellulose synthase-like proteins are a large family of proteins in plants thought to be processive polysaccharide β-glycosyltransferases. We have characterized an Arabidopsis mutant with a transposon insertion in the gene encoding AtCSLA7 of the CSLA subfamily. Analysis of the transmission efficiency of the insertion indicated that AtCSLA7 is important for pollen tube growth. Moreover, the homozygous insertion was embryo lethal. A detailed analysis of seed developmental progression revealed that mutant embryos developed more slowly than wild-type siblings. The mutant embryos also showed abnormal cell patterning and they arrested at a globular stage. The defective embryonic development was associated with reduced proliferation and failed cellularization of the endosperm. AtCSLA7 is widely expressed, and is likely to be required for synthesis of a cell wall polysaccharide found throughout the plant. Our results suggest that this polysaccharide is essential for cell wall structure or for signaling during plant embryo development.

Documentos Relacionados

• AtCSLD3, A Cellulose Synthase-Like Gene Important for Root Hair Growth in Arabidopsis1
• β-Cyanoalanine Synthase Is a Mitochondrial Cysteine Synthase-Like Protein in Spinach and Arabidopsis1
• Expression of cellulose synthase-like (Csl) genes in insect cells reveals that CslA family members encode mannan synthases
• Agrobacterium-Mediated Root Transformation Is Inhibited by Mutation of an Arabidopsis Cellulose Synthase-Like Gene1
• KOJAK encodes a cellulose synthase-like protein required for root hair cell morphogenesis in Arabidopsis