Aspectos epidemiológicos e laboratoriais (Eosinófilos e IgE total) em portadores de Schistosoma mansoni e Geohelmintos. / Epidemiological aspects and laboratory (Eosinophils and total IgE) in patients with Schistosoma mansoni and Geohelminths.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

This study aimed to evaluate laboratory aspects (total IgE and eosinophils) in patients with Schistosoma mansoni and geohelminths in three localities in Rio Largo, Alagoas. Coprological survey was conducted in 3030 subjects and examined two slides for each stool sample by the Kato-Katz technique, blood count to determine the absolute and relative number of eosinophilic and quantification of serum total IgE using the automated chemiluminescence system (Automated Chemiluminescence Systems) ACS: 180 SIEMENS, adapted to the System II and MAGIC LITE using commercial kits in 547 individuals from three localities infested by the snail Biomphalaria glabrata, close to the river Mundaú and environmental similarities. The subjects were divided into four groups, G1 (co-infected with S. mansoni and geohelminths): n = 115 (21.02%) with mean age 21.18 13.08 years, G2 (infected only by S. mansoni): n = 127 (23.22%) with mean age 29.59 16.54 years; G3 (infected only by geohelminths): n = 149 (27.24%) with mean age 23.99 15.52 years and G4 (negative for S. mansoni and geohelminths, the control group): n = 156 (28.52%) with mean age 25.44 14.60 years. The parasitic load of S. mansoni was quantified and classified according to intensity of infection by the number of eggs per gram of feces detected. The study was approved by the Ethics Committee of the Federal University of Alagoas and implemented in accordance with the resolution MS-CNS 196/96. For processing and data analysis used the software SPSS 11.5. In all analysis was considered the 5% level of significance. The descriptive statistical analysis of qualitative and quantitative variables were performed by the distribution of relative frequencies and absolute position measurements (mean) and dispersion (standard deviation). He applied the normality test, t test, analysis of variance oneway (ANOVA), chi-square test, Fisher exact test, Pearson and Spearman correlation and linear regression. Of the 3030 samples, 1,209 (39.90%) were positive for any helminth, and 679 (22.41%) positive for A. lumbricoides, 546 (18.02%) for T. Trichiura, 220 (7.26%) to Ancyostomatidae and 242 (8.0%) for S. mansoni. There was no statistically significant difference (p>0.05) of individuals positive for S. mansoni separated by gender, age and locality, the mean parasite burden was moderate and 111 epg (minimum 24 and maximum of 4,080 epg). Eosinophils increased significantly with increasing parasite load (p<0.05). The absolute and relative eosinophilia appear to have greater significance in relation to group coinfected, because presented more frequently than the other groups. There were no significant differences (p>0.05) between worm burden of S. mansoni and total IgE levels, nor between serum total IgE and age. Thus the total IgE test was not considered relevant to the diagnosis of Schistosomiasis mansoni. There was no correlation between markers of total IgE and geohelminth infections, whether they are co-infected with S. mansoni. The results suggest the importance of including eosinophil counts in epidemiological surveys, the complementation of the findings and parasitological diagnosis of schistosomiasis, contributing to more precise investigations focused on the control of schistosomiasis in endemic areas.

ASSUNTO(S)

ige tota esquistossomose mansônica carga parasitária ciencias da saude parasite load rio largo ige total schistosoma mansoni geohelminths s. mansoni, geohelmintos eosinophils eosinófilos schistosomiasis mansoni wide river (al)

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