Arsenic Speciation in Fish and Rice by HPLC-ICP-MS Using Salt Gradient Elution

AUTOR(ES)
FONTE

J. Braz. Chem. Soc.

DATA DE PUBLICAÇÃO

2018-08

RESUMO

This paper presents arsenic speciation studies by high-performance liquid chromatography with inductively coupled plasma-mass spectrometer (HPLC-ICP-MS) using a chromatography column containing a strong anion exchange stationary phase (Agilent Bio SAX G3154A/101) for determination of arsenobetaine (AsB), dimethylarsinic acid (DMA), AsIII, monomethylarsonic acid (MMA), and AsV. The best separation was achieved in 15 min, using a 4 min isocratic step with 1 mM (NH4)2HPO4 pH 10.2, followed by a 2 min salt gradient from 1 to 10 mM, which was kept for 4 min. Compared to the Food and Drug Administration (FDA) method for rice using the PRP-X-100 column, the Bio SAX method was quicker, provided at least equal separation of AsB from the next peak, and led to more symmetrical peaks. The linearity between 0.5 and 60 µg L-1 was shown. The method was tested with 10 µg L-1 fortified rice and fish samples extracted in ultrasound using methanol/water 1:1, and recoveries between 40 and 90% were obtained.

Documentos Relacionados