Arginina no processo de translocação bacteriana em modelo experimental de obstrução intestinal em ratos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Recent studies have shown that arginine reduces bacterial translocation in trauma and septic animal models whose diets were supplemented with it. The aim of this study was to evaluate the effects of arginine on bacterial translocation in an intestinal obstruction model in rats. Male Wistar rats (250g-350g) were randomized to six groups: Standard group (healthy animals), control group (sham operated + standard chow), OINT group (standard chow + IO), Arg 300mg/day group (standard chow supplemented with 300mg arginine/day + intestinal obstruction), Arg 600 mg/day group (standard chow supplemented with 600mg arginine/ day + IO) and Impact® group (standard chow supplemented with Impact® + IO). After 7 days of study initiation, animals were anesthetized. Terminal ileum was isolated and a ligature was placed around it. Escherichia coli labeled with 99m Technetium (99mTc-E.coli) was inoculated into the intestinal lumen. After 24 hours, blood, mesenteric lymph nodes (MLN), liver, spleen and lungs were collected for radioactivity determination. The yield labeling with 99mTc of E. coli was 98,44% (±1,08). Impact® group has showed higher caloric ingestion than other groups (p<0,05). Protein ingestion was higher in Arg 300 mg/day e Arg 600 mg/day (p<0,05). However, there was no significant difference in weight among the groups. Blood, MLN, liver, spleen and lungs of the OINT group showed significantly higher uptake of 99mTc-E.coli than the control group (p<0,05), validating the model of intestinal obstruction. The administration of arginine (300mg/day, 600mg/day or present in the enteral formula) reduced the level of bacterial translocation to the blood and all organs investigated when compared with the group OINT (p<0,05). In addition, the uptake of 99mTc-E.coli did not significantly differ between control and supplemented groups (p>0,05). The percentage of monocytes was significantly elevated in Arg 300mg/day and Impact® groups (p<0,05). In the last group, the phagocytosis of Candida albicans was significantly increased when compared to other groups (p>0,05). The results suggest that arginine was able to reduce the 99mTc-E.coli uptake in the blood and organs of the animals that received it as dietetic supplement.

ASSUNTO(S)

sistema gastrointestinal teses. microbiologia teses. alimentação enteral teses.

ACESSO AO ARTIGO

Documentos Relacionados