Análise histológica e por imunofluorescência de órgãos de camundongos para detecção de Klebsiella pneumoniae / Histological and immunofluorescent analysis of mouse organs for detection of Klebsiella pneumoniae

AUTOR(ES)

Antônio Frederico de Freitas Gomides

DATA DE PUBLICAÇÃO

2006

RESUMO

The objective of the study is to determine the presence of antigens of Klebsiella pneumoniae in tissues or organs of immunodepressed and nonimmunodepressed mice fed an enteric diet contaminated with K. pneumoniae and in the presence or absence of the prebiotics FOS and inuline. The liver and spleen of the animals were extracted on the sixth and seventh day of the experiment and analyzed with histological techniques of optic and fluorescent microscopy. For the indirect technique of immunoflourescence analysis using albino New Zealand rabbits, polyclonal antiserum containing antibodies for K. pneumoniae were produced. The antiserum yielded positive results in the serological tests for 14 isolates of Klebsiella sp. To detect K. pneumoniae, the indirect tests for immunofluorescence and staining with hematoxylin and eosin (H&E), toluidine blue, periodic acid Shiff (PAS), and Gram, showed no bacteria present in either the liver or spleen, nor did the histological analyses show evidence of alterations in the selected organs.

ASSUNTO(S)

translocação prebióticos prebiotics klebsiella pneumoniae translocation biologia geral klebsiella pneumoniae

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