AnÃlise comparativa em histograma da intensidade de fluorescÃncia de CD10 e CD19 em blastos leucÃmicos e hematogÃnias / Comparative analysis carried out through histograms of CD10 and CD19 fluorescence intensity in leukemic blasts and hematogones

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

17/06/2005

RESUMO

Hematogones are normal immature cells from bone marrow that are responsible for the production of the immune systemâs B cell lineage. The acute lymphoblastic leukemia (ALL) of precursors B cells represents one type of neoplastic transformation of hematogones. Due to their high similarity there are risks of erroneous interpretation consequently making it necessary use to complementary diagnostic techniques. The CD10 and CD19 antigens are expressed on both types of cells so, it is necessary use other monoclonal antibodies to identify malign or benign nature. In attempt to avoid the use of different antibodies we investigate possible differences in the expression of CD10 and CD19 in both cell types. We collected 36 samples of bone marrow from non-neoplastic patients as a control group. The age raged from 0 to 15 years with an average of 5 years. It was also collected 39 samples from patients with ALL of B cells. The age ranged from 0 to 14 years with an average of 6.6 years. We analyzed the differences between the fluorescence intensity concerning average, standard deviation, variation, inclination and kurtosis coefficients for the two markers. The individual values of each sample were compared with the intervals generated by the values of the control group: MEÂ2SD; MEÂ2.5SD and MEÂ3SD. It was possible to distinguish the groups with 89.7% and 75%; 79.5% and 100% and 71.8% e 100% of sensibility and specificity, respectively for the intervals. In conclusion, the expression of CD10 and CD19 antigens on blasts and hematogones is significantly different and may be useful in the differentiation of both cell types

ASSUNTO(S)

anatomia patologica e patologia clinica hematogÃnia antÃgenos cd10 e cd19 leucemia linfoblÃstica aguda hematogones cd10 and cd19 antigens acute lymphoblastic leukemia (all) citometria de fluxo leucemia

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