AlteraÃÃes fisiolÃgicas em Saccharomyces cerevisiae submetida a campo eletromagnÃtico estÃtico

AUTOR(ES)

Carine Carolina Wiesiolek

DATA DE PUBLICAÇÃO

2007

RESUMO

The objective of this study was to investigate the influence of static non-homogeneous magnetic fields on the physiology of the yeast S. cerevisiae growing in culture media containing glucose. The fermentations were driven into biorreators with magnetic rods produced with NdFeB (Neodymium-iron-boron) alloy, of 0.5â diameter and 0.25â thickness (model 27DNE3208 Magnet Sales &Manufacturing Company, Inc.USA) attached and into biorreatores without magnets. The applied static magnetic field no homogeneous had a field intensity of 220 mT. The yeast was obtained of the collection from the Departmento de AntibiÃticos of the Universidade Federal de Pernambuco, classified as UFPEDA-1012. The fermentations were accomplished in media containing yeast extract and glucose (5%) and for 24 hours of fermentation the microbiological conditions of the cultivation were monitored and sampling from the culture for the biomass growth evaluation, the dry weight measuring and the viable cell count (UFC / mL). The values of specific speed of cell growth (μ x) were calculated and were done image analyses, pH value measurements, determination of the ethanol concentration through gas chromatography, glucose level dosage by enzymatic method. The samples submitted to magnetic field showed increased biomass production, starting at 12 h of culture, when compared to the samples control. This increase was observed up to 181% in relation to the sample non-magnetized ones, evidencing that the magnetic field stimulates the biomass production in yeasts, as well as the increase in the number of viable cells, which increased of up to 4 times the value of the control samples. The analysis of the cell growth speed confirmed the previous discoveries with a control samples growth curve that showed a stationary phase whereas the experimental sample presented a prolongation of the exponential growth phase. This fact can be interpreted as an effect of the magnetic field on the acceleration of the cellular metabolism and could be used as a biotechnological technique in the enhancement and optimization of the biomass production. The magnetization produced outstanding influences on the results of the variables studied, showing that the applied magnetic field in S. cerevisiae, stimulated the proliferation and the biomass

ASSUNTO(S)

saccharomyces cerevisiae static magnetic field campo magnÃtico estÃtico saccharomyces cerevisiae physiology fisiologia fisiologia

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